Biosynthesis of high density lipoprotein by chicken liver: nature of nascent intracellular high density lipoprotein
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چکیده
منابع مشابه
Biosynthesis of High Density Lipoprotein by Chicken Liver: Nature of Nascent Intracellular High Density Lipoprotein
ABSTRACI Young chickens were administered L-[SH]leucine and after 10 or 30 min the livers were removed and fractionated into rough (RER) and smooth (SER) endoplasmic reticulum fractions and into light, intermediate, and heavy Golgi cell fractions. The labeled high density lipoprotein (HDL), contained within these intracellular organelles was isolated either by immunoprecipitation using rabbit a...
متن کاملBiosynthesis of high density lipoprotein by chicken liver: nature of nascent intracellular high density lipoprotein
Young chickens were administered L-[(3)H]leucine and after 10 or 30 min the livers were removed and fractioned into rough (RER) and smooth (SER) endoplasmic reticulum fractions and into light, intermediate, and heavy golgo cell fractions. The labeled high density lipoprotein (HDL), contained within these intracellular organelles was isolated either by immunoprecipitation using rabbit antiserum ...
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To study the assembly of newly synthesized lipids with apoprotein A1, we administered [2-3H]glycerol to young chickens and determined the hepatic intracellular sites of lipid synthesis and association of nascent lipids with apoprotein A1. [2-3H]glycerol was rapidly incorporated into hepatic lipids, reaching maximal levels at 5 min, and this preceded the appearance of lipid radioactivity in the ...
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To study the in vivo processing and secretion of Apolipoprotein A-I (Apo A-I), young chickens were administered individual L-[3H]amino acids intravenously and the time of intracellular transport of nascent Apo A-I from rough endoplasmic reticulum (RER) to the Golgi apparatus was measured. Within 3 to 9 min there was maximal incorporation of radioactivity into Apo A-I in both the RER and the Gol...
متن کاملCharacterization of nascent high density lipoprotein subfractions
Nascent high density lipoprotein (HDL) (1.063 < d < 1.21 g/ml) was isolated from recirculating rat liver perfusates and separated by heparin-Sepharose chromatography into a nonretained fraction (NR) and a fraction (R) that eluted with 0.5 M NaCI. Fractions NR and R contained 70% and 30% of the nascent HDL protein, respectively. ApoB-containing particles were removed from fraction R by chromatog...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1983
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.96.3.651